Sabin–Feldman dye test
Sabin–Feldman dye test | |
---|---|
Purpose | diagnose for toxoplasmosis. |
A Sabin–Feldman dye test is a serologic test to diagnose for toxoplasmosis. Patient serum is treated with Toxoplasma trophozoites and complement, and then incubated. After incubation, methylene blue is added. If anti-Toxo antibodies are present in the serum, the antibody-antigen complex activates complement to lyse the parasite membrane, Toxoplasma trophozoites are not stained (positive result); if there are no antibodies, trophozoites with intact membrane are stained and appear blue under microscope (negative result). The dilution of the test serum at which 50% of the tachyzoites are thin, distorted and colorless is reported as antibody titer of the test serum. The test is highly sensitive and specific with no false positives reported so far.[clarification needed]
Drawbacks of this test: 1. Difficulty in maintaining the live tachyzoites. 2. It detects immunoglobulin G(IgG) antibodies, hence cannot differentiate between recent or past infection. 3.False positive for Sarcocystis, Trypanosoma lewisi, Trichomonas vaginalis[clarification needed]
References
- Mosby's Medical, Nursing, & Allied Health Dictionary. Edition 5, 1998 p7B4A.
- v
- t
- e
- syphilis
- Venereal Disease Research Laboratory test
- rapid plasma reagin
- Wassermann test
- Fluorescent treponemal antibody absorption test
- Abelin reaction
- Rickettsia
- Helicobacter
- HelicoCARE direct
- Streptococcus
- Anti-streptolysin O
- toxoplasmosis
- Sabin–Feldman dye test
- malaria
This medical diagnostic article is a stub. You can help Wikipedia by expanding it. |
- v
- t
- e