NOX2

NADPH oksidaz 2 veya sitokrom b(558) alt-birimi beta veya Sitokrom B-245 ağır zincir, insanlarda NOX2 geni (diğer adıyla CYBB geni) tarafından kodlanan bir proteindir.[1] Bu protein, reaktif oksijen türlerini (ROS) oluşturan süper oksiti üreten bir enzimdir.

Fonksiyon

Nox2 veya Sitokrom b (-245), sitokrom b alfa (CYBA) ve beta (CYBB) zincirinden oluşur. Fagositlerin mikrobisidal oksidaz sisteminin birincil bileşeni olduğu düşünülür.

Nox2, NADPH oksidazın katalitik, zara bağlı alt birimidir. Flavositokrom b558 olarak bilinen heterodimeri oluşturan membrana sabitlenmiş p22 <i id="mwKA">phox'a</i> bağlanana kadar inaktiftir.[2] Aktivasyondan sonra, düzenleyici alt birimler p67phox, p47phox, p40phox ve tipik bir Rac olan GTPaz, plazma membranı veya fagozomal membran üzerinde NADPH oksidaz oluşturmak için komplekse dahil edilir.[3] Nox2'nin kendisi, iki hem grubunu bağlayan bir N-terminal transmembran domaininden ve FAD ve NADPH'ye bağlanabilen bir C-terminal domaininden oluşur.[4]

Kanıtlar, bu proteinin aortik ark, torasik ve abdominal aortta aterosklerotik lezyon gelişiminde önemli bir rol oynadığını göstermiştir.[5][6]

Etkileşimler

NoX2 Podosit TRPC6 kanalları ile doğrudan etkileşir.[7]

Kaynakça

  1. ^ "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)". 2 Kasım 2008 tarihinde kaynağından arşivlendi. 
  2. ^ "NADPH oxidases in Eukaryotes: red algae provide new hints!". Current Genetics. 49 (3): 190-204. Mart 2006. doi:10.1007/s00294-005-0044-z. PMID 16344959. 
  3. ^ "Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes". BMC Evolutionary Biology. 7: 178. Eylül 2007. doi:10.1186/1471-2148-7-178. PMC 2121648 $2. PMID 17900370. 
  4. ^ Aguirre (2010). "Nox enzymes from fungus to fly to fish and what they tell us about Nox function in mammals". Free Radical Biology and Medicine. 49 (9): 1342-1353. doi:10.1016/j.freeradbiomed.2010.07.027. PMC 2981133 $2. PMID 20696238. 
  5. ^ Sorescu (2002). "Superoxide production and expression of nox family proteins in human atherosclerosis". Circulation. 105 (12): 1429-35. doi:10.1161/01.cir.0000012917.74432.66. PMID 11914250. 
  6. ^ Chaubey (2013). "Nox2 is required for macrophage chemotaxis towards CSF-1". PLOS ONE. 8 (2): e54869. doi:10.1371/journal.pone.0054869. PMC 3562318 $2. PMID 23383302. 
  7. ^ "NOX2 interacts with podocyte TRPC6 channels and contributes to their activation by diacylglycerol: essential role of podocin in formation of this complex". American Journal of Physiology. Cell Physiology. 305 (9): C960-71. Kasım 2013. doi:10.1152/ajpcell.00191.2013. PMID 23948707. 

Konuyla ilgili yayınlar

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  • Nong Y, Kandil O, Tobin EH, Rose RM, Remold HG (Ocak 1991). "The HIV core protein p24 inhibits interferon-gamma-induced increase of HLA-DR and cytochrome b heavy chain mRNA levels in the human monocyte-like cell line THP1". Cellular Immunology. 132 (1): 10-6. doi:10.1016/0008-8749(91)90002-S. PMID 1905983. 
  • Dinauer MC, Pierce EA, Bruns GA, Curnutte JT, Orkin SH (Kasım 1990). "Human neutrophil cytochrome b light chain (p22-phox). Gene structure, chromosomal location, and mutations in cytochrome-negative autosomal recessive chronic granulomatous disease". The Journal of Clinical Investigation. 86 (5): 1729-37. doi:10.1172/JCI114898. PMC 296926 $2. PMID 2243141. 
  • Royer-Pokora B, Kunkel LM, Monaco AP, Goff SC, Newburger PE, Baehner RL, Cole FS, Curnutte JT, Orkin SH (1986). "Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location" (PDF). Nature. 322 (6074): 32-8. doi:10.1038/322032a0. hdl:2027.42/62926. PMID 2425263. 30 Mart 2012 tarihinde kaynağından arşivlendi (PDF). 
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  • Dinauer MC, Orkin SH, Brown R, Jesaitis AJ, Parkos CA (1987). "The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex". Nature. 327 (6124): 717-20. doi:10.1038/327717a0. PMID 3600768. 
  • Teahan C, Rowe P, Parker P, Totty N, Segal AW (1987). "The X-linked chronic granulomatous disease gene codes for the beta-chain of cytochrome b-245". Nature. 327 (6124): 720-1. doi:10.1038/327720a0. PMID 3600769. 
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  • Ariga T, Sakiyama Y, Matsumoto S (Ekim 1994). "Two novel point mutations in the cytochrome b 558 heavy chain gene, detected in two Japanese patients with X-linked chronic granulomatous disease". Human Genetics. 94 (4): 441. doi:10.1007/BF00201609. PMID 7927345. 
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  • Ariga T, Sakiyama Y, Tomizawa K, Imajoh-Ohmi S, Kanegasaki S, Matsumoto S (Haziran 1993). "A newly recognized point mutation in the cytochrome b558 heavy chain gene replacing alanine57 by glutamic acid, in a patient with cytochrome b positive X-linked chronic granulomatous disease". European Journal of Pediatrics. 152 (6): 469-72. doi:10.1007/BF01955051. PMID 8101486. 
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  • Kumatori A, Faizunnessa NN, Suzuki S, Moriuchi T, Kurozumi H, Nakamura M (Ekim 1998). "Nonhomologous recombination between the cytochrome b558 heavy chain gene (CYBB) and LINE-1 causes an X-linked chronic granulomatous disease". Genomics. 53 (2): 123-8. doi:10.1006/geno.1998.5510. PMID 9790760. 

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